Abstract
The influences of media consisting of various concentrations of glucose and peptone on the carbon and nitrogen availability of mycelia of Pleurotus ostreatus during flask shaking cultures were studied. PH, glucose concentration in broths and mycelial yields were varied with the C/N ratios of the media, but the influences of glucose concentrations on mycelial yields were greater than those of the C/N ratios. The contents of carbon and nitrogen in the mycelia were increased with a decrease of the C/N ratios. However, the increases of the yield in G4 (4% glucose) media were bigger than those in G2 (2% glucose) media. The availabilities of carbon by the mycelia were affected more with the glucose concentrations than the C/N ratios, while those of nitrogen were affected by the C/N ratios. The C/N ratios of the media produced both the highest mycelial yield and the nitrogen content of the mycelia were 24.2 in G2 media and 45.1 in G4 media, respectively.