2002 Volume 10 Issue 4 Pages 221-228
The optimal temperature and pH for the growth of F. obliqua mycelium were 30℃ and 5-7, respectively. When pieces of cultured fruit bodies were subcultured onto a new medium, new fruit bodies formed within a short period of time. The addition of mannitol, sorbitol, or trehalose increased the rate of the fruit body formation. By cultivating F. obliqua in bottles containing wheat flour-enriched Betula platyphylla sawdust and these saccharides, fruit bodies equaling 13% of the total weight of the culture media formed in 60days. We were also able to confirm that these fruit bodies formed spores. When these bottle cultures were studied for one year, the formation of sclerotia was confirmed.