Abstract
Double-stranded RNA (dsRNA) was detected in discolored strain of cultivated Flammulina velutipes, Shinano No. 6. First, mycelium was ground in phosphate buffer and virus particles were precipitated by PEG 8000 and NaCl added to the mycelial extract. After virus particles were phenol extracted, dsRNA became apparent in the agarose gel electrophoresis. A total of 3 dsRNA; S1 (size 1.4kb); S2 (size 1.6kb); and L1 (size 9.5kb) were present. This was the second example of dsRNA detected in the brown discolored fruit body of Junpakukei Enokitake. Thus, dsRNA could be causal agent for spontaneous brown discoloring of the cultivated F. velutipes.
