2003 Volume 11 Issue 4 Pages 159-164
Fuscoporia obliqua is a parasite on birches such as the white birch (Betula platyphylla), which is classified into white rot fungi. The sclerotium of F. obliqua is called Charga in Russian, whose extract with boiling water has several kinds of physiological activities, inhibition of HIV-1 protease, antitumor activity and hypoglycemic effects on plasma glucose. We examined several nutritional requirements of liquid culture to obtain much mycelia of F. obliqua. The optimum initial pH of liquid culture was 4.5 and the mycelia had an ability to grow in the pH range from 3.0 to 6.5. Trehalose was better for the mycelial growth than the other nine kinds of carbon sources and yielded the most mycelia with 3.0% concentration. Organic nitrogen sources were more effective than inorganic ones in nine kinds of materials. The most mycelia were obtained with yeast extract, whose optimum concentration was 0.6%. In this study, the fundamental conditions for the mass culture of F. obliqua mycelia were established.