The Cultivation of a Fibrinolytic Enzyme Producing Mushroom, Schizophyllum commune

Abstract

The artificial cultivation of Schizophyllum commune, which could produce fibrinolytic enzyme, was investigated. Schizophyllum commune produced a good yield of fruit bodies on a sawdust substrate. As a carbon source, monosaccharides proved to produce better growth than either disaccharides or polysaccharides. Organic nitrogen added to the substrate supported a better mycelial growth than inorganic nitrogen. The mushroom grew successfully on a medium using peptone as a nitrogen source. Thiamin was essential for mycelial growth and the addition of riboflavin stimulated the growth. Optimum temperature for fruiting was estimated to be at 15℃, however, the mycelia could grow well up to 35℃. The pH range between 7 and 11 was the most favorable for mycelial growth. The first flush at 14 days after the treatment of fruiting induction yielded about 10% of the corresponding wet weight of the spawned substrate which was composed of beech sawdust with rice bran.