Cloning and Sequence Analysis of a cDNA for the Gene FVFD30, Specifically Expressed During Fruiting Body Development in Flammulina velutipes

Abstract

A Flammulina velutipes CDNA library was constructed using mRNA from 7-day-old culture after fruiting induction, and a cDNA clone for the FVFD30 gene was selected by differential screening. The FVFD30 transcript was detected from 1 to 10day-old culture which forms primordia and particularly expressed at higher level in 7-day-old culture, but not in cultures before fruiting induction, several days after primordium formation and after that. It is suggested that the FVFD30 is involved in the initial stages of fruiting body development in F. velutipes. The FVFD30 cDNA contains an open reading frame encoding a putative protein of 319 amino acid residues (MW=34,592), which might belong to the oxidoreductase family.