Abstract
Fruit body production from a submerged culture mycelia of Favolus arcularius was examined. A glass bottle, 3 L in capacity, was used as the culture vessel, which contained 2 L of liquid medium composed of maltose and peptone as carbon and nitrogen sources, respectively. A large number of small pieces of mycelia was inoculated, and incubated at 27℃ for 120 h with agitation. The mycelial pellets were collected by filtration, placed into Petri dishes, and then the culture filtrates were poured to immerse up to a half of the height of mycelium pellets. The dishes were incubated to induce fruiting at 27℃ under light exposure. By incubating the dishes for 1-2 d, the aerial mycelia appeared on the surface of mycelium pellets. It grew primordia, that would develop into mature fruit bodies. However, the filamentous mycelia by submerged culture did not exhibit fruit body formation during incubation for 2 weeks. The optimal pellet size was estimated to be around 10 mm in diameter. One pellet could produce only one mature fruit body, although two or more numbers of primordia could arise on a pellet. Fruiting was completed within 10 days after the treatment of mycelium pellets for fruit body production.
