Abstract
Amylase productions from Lyophyllum shimeji, a ectomycorrhizal fungus, in a partly modified matsutake liquid medium were examined using fruit-body forming or non-forming strains on a medium consisting of barley grains. The enzyme activities were assayed by Somogyi-Nelson (amylase), iodine-potassium iodide (α -amylase) and F-kit glucose method (glucoamylase) with soluble starch as a substrate. These activities in culture filtrate were detected to have relatively high values at 15 days after inoculation, and the activities reached a maximum activity at 37〜43 days after inoculation. The activity varied considerably among the test strains, but amylase and glucoamylase in the fruit-body forming strains showed higher activity level than those of the fruit-body non-forming strains. The active fractions obtained from the DEAE-Toyopearl column chromatography were identified. As the results, a glucoamylase and a endo-type amylase activity have been detected from the active fractions. These amylase activities were remarkably activated by the presence of Ca^<2+> ion, but showed no effect on thermal stability.
