Abstract
Extracellular amylase productions during the vegetative mycelial growth from Tricholoma matsutake, an ectomycorrhizal fungus, in a partly modified matsutake liquid medium were examined. The amylase activities in the culture filtrate (α-and gluco-amylase) were detected to have relatively high values at 40 days after inoculation, and the activities reached a maximum activity at 80 days after inoculation. α-Amylase showed higher activity than glucoamylase in each strain. Using several kinds of starches purified from barley (5 kinds), corn, sweet potato and potato as the substrates, amylase activities in the culture filtrate (60 days after inoculation) of T. matsutake were assayed. As the results, the enzyme showed the highest activity toward the starch prepared from "Amagi" and "Ichibanboshi" strain in the 「nonwa xy」 group of barley of all the starches. These activities were obtained with about a 2.0-fold higher value than in the control (potato). Therefore, the addition of potato and yam to the culture medium increased the dry weight of the mycelia 4.8〜5.6 times compared to the control (without addition), and increased the value of amylase activity 1.9〜2.6 times compared to the control. The positive correlation of the vegetative mycelial growth with amylase activity was detected.
