Abstract
We evaluated bronopol as a practical alternative anti-fungal agent to malachite green for use in hatcheries. Repeated daily exposure, from just after fertilization to the stage showing eye development, to 50 ppm and 100 ppm bronopol for 30 min showed an efficacy compared to the 0 ppm control for the inhibition of fungal infections in ayu Plecoglossus altivelis eggs. The 100 ppm bronopol treatment groups showed control of fungal infection up to the stage of eye development in eggs. Significant differences in terms of the number of hatched fish were seen between the 0 ppm control and treatments at 50 and 100 ppm bronopol.
