Investigation of Conditions Optimum for Spontaneous Rosette Formation by Lymphocytes of Yellowtail, Seriola quinqueradiata

Abstract

Various factors involved in spontaneous rosette formation with erythrocytes by peripheral blood lymphocytes of yellowtail, Seriola quinqueradiatawere investigated. The percentage of the rosette forming lymphocytes was higher with sheep red blood cells (SRBCs) than either with rabbit red blood cells or bovine red blood cells. Binding of SRBCs to lymphocytes was enhanced after treating SRBCs with 2-aminoethylisothiouronium bromide and trypsin but not with neuraminidase. There were no significant differences among the percentage of rosette forming lymphocytes incubated at 4, 25 and 37°C within 3 h of incubation. After 3 h, the percentage began to decrease and rosette could be detected only at 4°C after 24 h. Higher value of rosette formation was obtained when the lymphocytes were incubated in L-15 medium non-supplemented with fetal calf serum.