Studies on the Intravascular Antigen-Antibody Reaction of Thrombocyte : IV. Histological Changes of Spleen in Active Anaphylaxis

Abstract

Continuing the investigation of the previous report, the author reported on the macroscopical and histological changes in the spleen caused on the active anaphylaxis, which was a part of studies on the intravascular antigen-antibody reaction of thrombocyte. On the experimental method, normal rabbits were immunized frequently with the normal dog platelets. Thereafter, rabbits were operated by means of laparotomy and the provocative injection was administered into the splenic artery with the normal dog platelets (Experimental Group A). On the other hand, the normal dog platelets were injected into the marginal ear vein of immunized rabbits (Experimental Group B) Rabbits were sacrificed and autopsied periodically from twenty-four hours to two weeks after the provocative injection and the macroscopical and histological changes of spleen were examined. In the experimental group A, no remarkable macroscopical changes were found. In the histlogical observation, the following remarkable changes were found: hyperplasia of lymph follicles, congestion and dilatation of sinus, swelling of the sinus endothelial cells, inflammatory leukocytic infiltration, erythro- and thrombophagocytosis, hemosiderosis, appearance of megakaryocytes in the sinus, some foci of extramedullary hemopoiesis and edema in the trabecular. In the experimental group B, macroscopical changes were not found. Histological changes were observed similar to those in the experimental group A, but degree of the damages were slight.