Roadkill associated with transportation infrastructure exerts a negative impact on the natural environment and driving; hence, it should be prevented to conserve wildlife and promote safe driving. In this study, drivers in Tottori Prefecture were surveyed regarding their recognition and attitudes toward roadkill. The survey was conducted in September 2020, and online questionnaires were collected from 500 respondents aged 20 to 60. A total of 406 respondents (81.2%) answered that they know what roadkill is and that they had seen it most commonly on roads. More than 90% of those who were aware of roadkill felt that roadkill should not occur, primarily out of sympathy for animals and traffic accident danger. In addition, there was a difference in consciousness depending on the animal species. Roadkill prevention consciousness for large mammals stemmed from a concern to avoid driving dangers, while the reason given for preventing endangered species from becoming roadkill was the need for conservation. Many respondents thought that amphibians, reptiles, and insects are small and collisions are inevitable. Also, more than 80% of respondents thought that roadkill is a problem for both humans and animals. This information on drivers' attitudes toward roadkill prevention in Tottori Prefecture is valuable as a resource for roadkill policies in the future.