Escherichia coli Transformant Expressing the Glucose Dehydrogenase Gene from Bacillus megaterium as a Cofactor Regenerator in a Chiral Alcohol Production System

Michihiko KATAOKA; Luh Poni Sri ROHANI; Masaru WADA; Keiko KITA; Hideshi YANASE; Itaru URABE; Sakayu SHIMIZU

doi:10.1271/bbb.62.167

Microbiology & Fermentation Industry Notes

Escherichia coli Transformant Expressing the Glucose Dehydrogenase Gene from Bacillus megaterium as a Cofactor Regenerator in a Chiral Alcohol Production System

Michihiko KATAOKA, Luh Poni Sri ROHANI, Masaru WADA, Keiko KITA, Hideshi YANASE, Itaru URABE, Sakayu SHIMIZU

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Keywords: glucose dehydrogenase, Bacillus megaterium, NADPH regeneration, aldehyde reductase, asymmetric reduction

JOURNAL FREE ACCESS

1998 Volume 62 Issue 1 Pages 167-169

DOI https://doi.org/10.1271/bbb.62.167

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Abstract

Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP⁺ and glucose, similarly to commercially available GDH.

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