Compensation for D-Glutamate Auxotrophy of Escherichia coli WM335 by D-Amino Acid Aminotransferase Gene and Regulation of murI Expression

Abstract

  D-Glutamate, an indispensable component of peptidoglycans of bacteria, is provided by glutamate racemase in E. coli cells. Compensation for D-glutamate auxotrophy of E. coli WM335 cells lacking the glutamate racemase gene, murI, with the D-amino acid aminotransferase gene suggests the presence of a threshold concentration for the D-glutamate required by E. coli cells, as well as a regulation system for murI expression.