Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Physical Mapping of the Linear Plasmid pSLA2-L and Localization of the eryAI and actI Homologs
Haruyasu KINASHIShingo FUJIIAkira HATANIToru KUROKAWAHidenori SHINKAWA
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1998 Volume 62 Issue 10 Pages 1892-1897

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Abstract

  The 200-kb linear plasmid pSLA2-L was suggested to be involved in the production of lankamycin and lankacidin in Streptomyces rochei 7434AN4. In this study, we have constructed a physical map for 23 PstI fragments of pSLA2-L, the sum of which was 206 kb. Detailed restriction maps for both ends of pSLA2-L revealed the presence of terminal inverted repeats, the size of which was found to be 2.1 kb by cloning and sequencing of the end-points. Hybridization experiments using two polyketide biosynthetic genes, eryAI and actI, located their homologous regions on PstI fragments A and I, respectively.

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© 1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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