Structural Study on a Sulfated Polysaccharide-peptidoglycan Complex Produced by Arthrobacter sp.

Abstract

  The structure of a sulfated polysaccharide-peptidoglycan complex (SP-PG) produced by Arthrobacter sp. was analyzed by NMR spectroscopy. In addition, oligosaccharide fragments of the SP-PG-L obtained by HF degradation were analyzed by NMR spectroscopy. These findings indicated that the sulfated polysaccharide (SP) contains a repeating unit composed of two galactofuranosides and a glucopyranoside. The main chain of the trisaccharide is [→6)β-D-Galf(1→6)-β-D-Galf(1→]_n, with β-D-Glcp linked to one of the Galfs through a (1→2) linkage. The sulfated positions of the trisaccharide were identified as C-3 and C-5 of the β-glucosylated Galf residues, and C-2 or C-3 of the other Galf residue.