Tryptophan Pyrolysis Products, Trp-P-1 and Trp-P-2 Induce Apoptosis in Primary Cultured Rat Hepatocytes

Abstract

  The cytotoxicity of heterocyclic amines, dietary carcinogens derived from cooked foods, to primary cultured rat hepatocytes was studied. A tryptophan pyrolysis product, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) was the most cytotoxic of 11 compounds tested. Trp-P-1 was found to induce apoptosis as measured by morphological changes in nuclear chromatin and internucleosomal DNA fragmentation. 3-Amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) showed a moderate apoptotic effect, and other compounds had a similar but weaker effect.