Non-thermal Effect of a Ceramics Radiation on a Yeast Glucose-6-phosphate dehydrogenase

Abstract

  Non-thermal effect of a ceramics radiation on glucose-6-phosphate dehydrogenase has been investigated using the enzyme, glucose-6-phosphate and NADP⁺ separately irradiated at 10°C by a ceracompo R plate and a ceramics un-sewed cloth (sheet). The K_m for glucose-6-phosphate was increased 20% after 6 h of irradiation by the plate, but the V_max/K_m was decreased 24%. After 3 h of irradiation by the sheet, the K_m was increased 17%, but after 6 h of irradiation it was decreased 11%. The 3 h of irradiation by the sheet slightly increased both enthalpy and entropy changes of the reaction, but the 6 h of irradiation significantly decreased them. Both thermodynamic parameters in the activated state were increased by the sheet irradiation. The promotion energy for both formations of the enzyme-substrate and their activated complex depended on enthalpy. The different effects of two ceramics radiators on G6PDH activity were discussed.