Enzymatic Synthesis of 6-O-α-<small>D</small>-Galactopyranosyl-1-deoxynojirimycin Using α-Galactosidase from Green Coffee Beans

Nam Soo PAEK; Dae Jung KANG; Hong Sub LEE; Jung Joon LEE; Yong Jin CHOI; Tae Han KIM; Kee Won KIM

doi:10.1271/bbb.62.588

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biological Chemistry Notes

Enzymatic Synthesis of 6-O-α-D-Galactopyranosyl-1-deoxynojirimycin Using α-Galactosidase from Green Coffee Beans

Nam Soo PAEK, Dae Jung KANG, Hong Sub LEE, Jung Joon LEE, Yong Jin CHOI, Tae Han KIM, Kee Won KIM

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Keywords: 1-deoxynojirimycin, α-galactosidase, 6-O-α-D-galactopyranosyl-1-deoxynojirimycin

JOURNAL FREE ACCESS

1998 Volume 62 Issue 3 Pages 588-589

DOI https://doi.org/10.1271/bbb.62.588

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Abstract

A transgalactosylation reaction from p-nitrophenyl-α-D-galactopyranoside to 1-deoxynojirimycin was done using α-galactosidase [EC 3.2.1.22] from green coffee beans. The enzyme formed 6-O-α-D-galactopyranosyl-1-deoxynojirimycin as the major product.

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