Molecular Cloning and Sequence Analysis of Catalase cDNA from Green Pepper Seedlings Elicited with Arachidonic Acid

Abstract

  We isolated a cDNA encoding catalase from green pepper seedlings elicited with arachidonic acid, based on the amino acid sequences of the purified protein. The nucleotide sequence of the isolated cDNA contained a single open reading frame predicted to encode 492 amino acid residues with a calculated molecular mass of 56439.0 daltons. The deduced amino acid sequence contained the amino acid sequences found by sequencing of the peptides. The total deduced amino acid sequence showed high similarity with those of the other plant catalases reported so far and was found to possess the peroxisomal targeting sequence conserved among plant catalases. Transcription of the catalase gene in green pepper seedlings was found to be induced by treatment with arachidonic acid.