Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities

Koji HIRATA; Yoichi ASO; Shoji YASUDA; Masatsune ISHIGURO

doi:10.1271/bbb.62.807

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biological Chemistry Notes

Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities

Koji HIRATA, Yoichi ASO, Shoji YASUDA, Masatsune ISHIGURO

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Keywords: α-fucosidase, apple snail, isoform, Pomacea canaliculata

JOURNAL FREE ACCESS

1998 Volume 62 Issue 4 Pages 807-810

DOI https://doi.org/10.1271/bbb.62.807

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Abstract

Two isoforms, F1 (pI 4.7) and F2 (pI 4.9), of α-fucosidase were purified from the viscera of Pomacea canaliculata. The thermostability of F1 was higher than that of F2. Both were 260-kDa proteins containing polypeptides (55 and 52 kDa) and sugar. There were small differences between F1 and F2 in the optimum conditions for the enzyme reaction and pH stability.

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