Oxidation of Two Pregnane Steroids Catalyzed by the Fungus Cephalosporium aphidicola

Abstract

  Two pregnane steroids, pregnane (1) and 3β-hydroxypregnane (2), were oxidized by fermentation with the fungus Cephalosporium aphidicola. The fermentation of pregnane (1) yielded 3β-hydroxypregnane (2) and 3β, 6β,11α-trihydroxypregnane (3), while that of 3β-hydroxypregnane (2) afforded 6β,11α-dihydroxypregn-3,20-dione (4), 3β,6β,15α-trihydroxypregn-20-one (5) and 3β,5α,11α-trihydroxypregn-20-one (6). The metabolites are characterized by detailed physical and spectroscopic studies.