A Rapid Method for Transformation of Carrot (Daucus carota L.) by Using Direct Somatic Embryogenesis

Abstract

  Several genes that may be involved in embryogenesis have been isolated from somatic embryos of carrot by many workers. However, the function of these genes has not been discovered yet. As the first step toward finding the function of these genes, we established a rapid and efficient method for transformation of carrot by using direct embryogenesis from hypocotyl segments treated with 2,4-dichlorophenoxyacetic acid (2,4-D) for a short period.