Brachyury Regulatory Region Active in Embryonal Carcinoma P19 Cells

Abstract

  Brachyury (T) is involved in mesoderm induction during early mouse development. We analyzed the region regulating expression of T in embryonal carcinoma P19 cells, which differentiate into mesoderm derivatives in vitro. Transfection of plasmids encoding reporter genes under the control of the 5′-flanking region showed positive regulatory elements between -298 and -129 bp are responsible for driving T expression in mesodermal cells.