Cell Layer-Specific Accumulation of Anthocyanins in Response to Gibberellic Acid in Tepals of Hyacinthus orientalis

Abstract

  Anthocyanin-producing cells in the tepals of flower buds of Hyacinthus orientalis were investigated. In field-grown flowers, anthocyanins were only produced in L2 cells (cells in the sub-epidermis) of tepals. However, when early-stage flower buds were cultured on medium containing GA₃, anthocyanins were produced not only in L2 cells but also in L1 cells (cells in the epidermal layer) of tepals. The same anthocyanins were produced in tepals of both field-grown flowers and flower buds cultured in vitro.