Isolation and Characterization of the Actin Gene from the Cellulolytic Fungus Humicola grisea and Analysis of Transcription Levels of Actin and Cellulase Genes

Shou TAKASHIMA; Akira NAKAMURA; Makoto HIDAKA; Haruhiko MASAKI; Takeshi UOZUMI

doi:10.1271/bbb.63.1091

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Microbiology & Fermentation Technology Notes

Isolation and Characterization of the Actin Gene from the Cellulolytic Fungus Humicola grisea and Analysis of Transcription Levels of Actin and Cellulase Genes

Shou TAKASHIMA, Akira NAKAMURA, Makoto HIDAKA, Haruhiko MASAKI, Takeshi UOZUMI

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Keywords: actin, cellulase, Humicola grisea

JOURNAL FREE ACCESS

1999 Volume 63 Issue 6 Pages 1091-1095

DOI https://doi.org/10.1271/bbb.63.1091

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Abstract

An actin gene was isolated from the cellulolytic fungus Humicola grisea. The gene structure, which has 5 introns in the coding region, is similar to those of the so far cloned fungal actin genes. But there are some differences in intron sizes and codon usage. Transcription levels of actin and cellulase genes were also investigated.

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