Purification and Characterization of Recombinant Mortierella vinacea α-Galactosidases I and II Expressed in Saccharomyces cerevisiae

Hajime SHIBUYA; Hideyuki KOBAYASHI; Shigeki YOSHIDA; Satoshi KANEKO; Gwi Gun PARK; Isao KUSAKABE

doi:10.1271/bbb.63.1096

Biochemistry & Molecular Biology Notes

Purification and Characterization of Recombinant Mortierella vinacea α-Galactosidases I and II Expressed in Saccharomyces cerevisiae

Hajime SHIBUYA, Hideyuki KOBAYASHI, Shigeki YOSHIDA, Satoshi KANEKO, Gwi Gun PARK, Isao KUSAKABE

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Keywords: α-galactosidase, Mortierella vinacea, expression, Saccharomyces cerevisiae

JOURNAL FREE ACCESS

1999 Volume 63 Issue 6 Pages 1096-1099

DOI https://doi.org/10.1271/bbb.63.1096

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Abstract

The cDNAs coding for Mortierella vinacea α-galactosidases I and II were expressed in Saccharomyces cerevisiae under the control of the yeast GAL10 promoter. The recombinant enzymes purified to homogeneity from the culture filtrate were glycosylated, and had properties identical to those of the native enzymes except for improving the heat stability of α-galactosidase II and decreasing the specific activities of both enzymes.

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