Dephosphorylation of Specific Proteins during Induction of Senescence in Immortal human Fibroblasts Expressing Thermolabile SV40 T Antigen

Michihiko FUJII; Atsuhiro JOGUCHI; Hideki OGINO; Kazuhiko NAKABAYASHI; Dai AYUSAWA

doi:10.1271/bbb.63.1116

Biochemistry & Molecular Biology Notes

Dephosphorylation of Specific Proteins during Induction of Senescence in Immortal human Fibroblasts Expressing Thermolabile SV40 T Antigen

Michihiko FUJII, Atsuhiro JOGUCHI, Hideki OGINO, Kazuhiko NAKABAYASHI, Dai AYUSAWA

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Keywords: protein kinase inhibitor, protein phosphatase, H-9, senescence, human cell

JOURNAL FREE ACCESS

1999 Volume 63 Issue 6 Pages 1116-1118

DOI https://doi.org/10.1271/bbb.63.1116

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Abstract

Particular protein kinase inhibitors block a senescence-like phenomenon in SVts8 cells induced by a shift up in temperature. We characterized cellular proteins with affinity chromatography using one such inhibitor as a ligand. Two proteins of 56 and 100 kDa were found to be dephosphorylated specifically, probably due to induction of a protein phosphatase activity(s).

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