Simultaneous Determination of Lipid Hydroperoxides by HPLC-Post Column Systems

Kazuaki AKASAKA; Hirotaka OHTA; Yoshimi HANADA; Hiroshi OHRUI

doi:10.1271/bbb.63.1506

Analytical Chemistry Notes

Simultaneous Determination of Lipid Hydroperoxides by HPLC-Post Column Systems

Kazuaki AKASAKA, Hirotaka OHTA, Yoshimi HANADA, Hiroshi OHRUI

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Keywords: lipid hydroperoxide, diphenyl-1-pyrenylphosphine, semi-micro high-performance liquid chromatography, post-column high-performance liquid chromatography

JOURNAL FREE ACCESS

1999 Volume 63 Issue 8 Pages 1506-1508

DOI https://doi.org/10.1271/bbb.63.1506

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Abstract

Two HPLC-post column systems, a conventional-size system and a semi-micro system, for the simultaneous determination of lipid hydroperoxides were developed. The hydroperoxides of free fatty acids, phosphatidylcholines, triacylglycerols and cholesterol esters were individually determined at the pmol level with good reproducibility by using gradient elution and post-column detection with diphenyl-1-pyrenylphosphine.

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