Ultraviolet Spectrometric Determination of Neutral Monosaccharides by HPLC with Ethanolamine

Akihiro NAKAMURA; Chitoshi HATANAKA; Yasunori NAGAMATSU

doi:10.1271/bbb.64.178

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Analytical Chemistry Note

Ultraviolet Spectrometric Determination of Neutral Monosaccharides by HPLC with Ethanolamine

Akihiro NAKAMURA, Chitoshi HATANAKA, Yasunori NAGAMATSU

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Keywords: neutral monosaccharide, ethanolamine, UV detection, HPLC, pectin

JOURNAL FREE ACCESS

2000 Volume 64 Issue 1 Pages 178-180

DOI https://doi.org/10.1271/bbb.64.178

[Correction] Biosci. Biotechnol. Biochem. Vol.64 (2000) No.2 p.E2

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Abstract

The reaction between ethanolamine and sugars in a borate solution gave intense UV absorption at 310 nm. By adding ethanolamine in the mobile phase, simple and sensitive ion-exchange HPLC of sugar-borate complexes was achieved. This method can be used to measure as little as 100 pmole of seven monosaccharides from pectic polysaccharides.

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