Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Tributyrin Specifically Induces a Lipase with a Preference for the sn-2 Position of Triglyceride in Geotrichum sp. FO401B
Yasuhide OTATakeshi SAWAMOTOMasaki HASUO
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2000 Volume 64 Issue 11 Pages 2497-2499

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Abstract

  The extracellular Lipases A and C produced by Geotrichum sp. FO401B have a preference for the sn-1,3 and sn-2 positions of triglyceride, respectively. Total production of these lipases was increased by plant oils and tributyrin. Butyl Toyopearl column chromatography demonstrated that only Lipase C was produced in the presence of tributyrin. Lipase C hydrolysed natural fats except sardine oil preferentially at the sn-2 position, but it showed little stereoselectivity for triolein.

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© 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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