Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene

Teisuke TAKITA; Naofumi SHIMIZU; Tokuo SUKATA; Satoshi HASHIMOTO; Eriko AKITA; Takuya YOKOTA; Nobuyoshi ESAKI; Kenji SODA; Kuniyo INOUYE; Ben’ichiro TONOMURA

doi:10.1271/bbb.64.432

Biochemistry & Molecular Biology Notes

Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene

Teisuke TAKITA, Naofumi SHIMIZU, Tokuo SUKATA, Satoshi HASHIMOTO, Eriko AKITA, Takuya YOKOTA, Nobuyoshi ESAKI, Kenji SODA, Kuniyo INOUYE, Ben’ichiro TONOMURA

著者情報

キーワード: Bacillus stearothermophilus, gene cloning, gene expression, lysyl-tRNA synthetase

ジャーナルフリー

2000 年 64 巻 2 号 p. 432-437

DOI https://doi.org/10.1271/bbb.64.432

詳細

抄録

The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA1503 was cloned and sequenced. The gene consists of 1485 bp nucleotides commencing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids. The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promoter.

責任著者(Corresponding author)

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