A Simple and Rapid Method for the Preparation of a Cell-free Extract with CCAAT-Binding Activity from Filamentous Fungi

Masashi KATO; Kojiro HAYASHI; Tetsuo KOBAYASHI; Norihiro TSUKAGOSHI

doi:10.1271/bbb.64.455

Biochemistry & Molecular Biology Notes

A Simple and Rapid Method for the Preparation of a Cell-free Extract with CCAAT-Binding Activity from Filamentous Fungi

Masashi KATO, Kojiro HAYASHI, Tetsuo KOBAYASHI, Norihiro TSUKAGOSHI

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Keywords: filamentous fungi, CCAAT-binding protein, cell free extracts, electrophoretic mobility shift assays

JOURNAL FREE ACCESS

2000 Volume 64 Issue 2 Pages 455-457

DOI https://doi.org/10.1271/bbb.64.455

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Abstract

A simple and rapid method for the preparation of a cell-free extract with the CCAAT-binding activity was established with Aspergillus nidulans as a model fungus. Proteins were extracted with 6 M guanidine hydrochloride directly from mycelia and renatured by dialysis. This method was found applicable to other filamentous fungi such as Aspergillus oryzae and Trichoderma viride.

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