Isolation and Some Properties of a Serine Protease from the Fruits of Cudrania cochinchinensis (Lour.) Kudo et Masam.

Tetsuya UCHIKOBA; Kazunari ARIMA; Masayuki SHIMADA; Hiroo YONEZAWA; Mokoto KANEDA

doi:10.1271/bbb.64.623

Biochemistry & Molecular Biology Notes

Isolation and Some Properties of a Serine Protease from the Fruits of Cudrania cochinchinensis (Lour.) Kudo et Masam.

Tetsuya UCHIKOBA, Kazunari ARIMA, Masayuki SHIMADA, Hiroo YONEZAWA, Mokoto KANEDA

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Keywords: Cudrania cochinchinensis, fruits, Moraceae, serine protease

JOURNAL FREE ACCESS

2000 Volume 64 Issue 3 Pages 623-624

DOI https://doi.org/10.1271/bbb.64.623

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Abstract

An endopeptidase (Cudrania protease) with a molecular mass of 76 kDa has been purified from the fruits of Cudrania cochinchinensis (Lour.) Kudo et Masam. The enzyme was stable between pH 6 and 10 at 30°C for 60 min. The enzyme activity was inhibited by diisopropyl fluorophosphate, chymostatin, and aprotinin, but not by EDTA or pepstatin. These results indicated that the enzyme was a serine protease.

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