Hybridization and Breeding of the Benomyl Resistant Mutant, Trichoderma harzianum Antagonized to Phytopathogenic Fungi by Protoplast Fusion

Kihachiro OGAWA; Naoto YOSHIDA; Wanwilai GESNARA; Crispinus A. OMUMASABA; Chiradej CHAMUSWARNG

doi:10.1271/bbb.64.833

Microbiology & Fermentation Technology Notes

Hybridization and Breeding of the Benomyl Resistant Mutant, Trichoderma harzianum Antagonized to Phytopathogenic Fungi by Protoplast Fusion

Kihachiro OGAWA, Naoto YOSHIDA, Wanwilai GESNARA, Crispinus A. OMUMASABA, Chiradej CHAMUSWARNG

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Keywords: protoplast fusion, antagonistic fungus, Trichoderma harzianum, phytopathogenic fungi

JOURNAL FREE ACCESS

2000 Volume 64 Issue 4 Pages 833-836

DOI https://doi.org/10.1271/bbb.64.833

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Abstract

A diploid strain obtained from heterokaryons of Trichoderma harzianum by protoplast fusion grew on minimal medium containing 100 ppm benomyl. This strain inhibited the growth of the phytopathogenic fungus Fusarium oxysporum f. sp. raphani on paired cultures and also protected against radish yellows and a drop in germination induced by F. oxysporum f. sp. raphani.

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