Purification and Partial Characterization of a Basic Xylanase Produced by Thermoalkaliphilic Bacillus sp. Strain TAR-1

Abstract

  A basic xylanase was purified from the culture supernatant of thermoalkaliphilic Bacillus sp. strain TAR-1. Its molecular mass and isoelectric point were 23 kDa and >pH 9.3, respectively. The enzyme showed a broad pH profile and was optimally active at 70°C. Analyses of xylan-degradation products and N-terminal amino acid sequence revealed that the enzyme would be a family 11/G endoxylanase.