A Simple and Rapid Extraction of High Molecular Weight Chromosomal DNA from Bacillus subtilis Protoplasts for Cosmid Cloning and Interspecific Transformation

Takashi AKAMATSU; Hisataka TAGUCHI; Hirosuke OKADA

doi:10.1271/bbb.64.1082

Biochemistry & Molecular Biology Notes

A Simple and Rapid Extraction of High Molecular Weight Chromosomal DNA from Bacillus subtilis Protoplasts for Cosmid Cloning and Interspecific Transformation

Takashi AKAMATSU, Hisataka TAGUCHI, Hirosuke OKADA

著者情報

キーワード: Bacillus subtilis, DNA, extraction, high-molecular-weight, transformation

ジャーナルフリー

2000 年 64 巻 5 号 p. 1082-1083

DOI https://doi.org/10.1271/bbb.64.1082

詳細

抄録

After conversion of Bacillus subtilis vegetative cells to protoplasts, a simple and rapid method for extracting high-molecular-weight chromosomal DNA was devised with the inclusion of bovine serum albumin and phenol-chloroform treatments. The DNA sample thus prepared was the size of 100-450 kb and could be used for cosmid cloning and interspecific transformation.

責任著者(Corresponding author)

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