Isolation and Characterization of Low-indole-3-acetic Acid-producing Mutants from Bradyrhizobium elkanii

Ken YAGI; Taku MATSUMOTO; Tetsuya CHUJO; Hideaki NOJIRI; Toshio OMORI; Kiwamu MINAMISAWA; Makoto NISHIYAMA; Hisakazu YAMANE

doi:10.1271/bbb.64.1359

Organic Chemistry Regular Papers

Isolation and Characterization of Low-indole-3-acetic Acid-producing Mutants from Bradyrhizobium elkanii

Ken YAGI, Taku MATSUMOTO, Tetsuya CHUJO, Hideaki NOJIRI, Toshio OMORI, Kiwamu MINAMISAWA, Makoto NISHIYAMA, Hisakazu YAMANE

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Keywords: Bradyrhizobium elkanii, biosynthesis of indole-3-acetic acid, indole-3-pyruvic acid decarboxylase, Tn5 mutagenesis

JOURNAL FREE ACCESS

2000 Volume 64 Issue 7 Pages 1359-1364

DOI https://doi.org/10.1271/bbb.64.1359

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Abstract

We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.

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