Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Notes
Phylogenetic Analysis of Methanogens in Sheep Rumen Ecosystem and Detection of Methanomicrobium mobile by Fluorescence In Situ Hybridization
Kazuhiro YANAGITAYoichi KAMAGATAMamoru KAWAHARASAKIToshihiko SUZUKIYutaka NAKAMURAHajime MINATO
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2000 Volume 64 Issue 8 Pages 1737-1742

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Abstract

  The population of methanogens in the sheep rumen microbial ecosystem was studied by using 16S rDNA cloning analysis, epifluorescence microscopy (which detects autofluorescence of a specific cofactor F420 in methanogens) and the 16S rRNA-targeted in situ hybridization technique. The 16S rDNA clone libraries were constructed by PCR amplification with an Archaea-specific primer set and partial sequencing of the clonal 16S rDNAs was done. Phylogenetic analysis indicated that the clones were affiliated with Methanomicrobium mobile, Methanobrevibacter ruminantium and Methanobrevibacter smithii. Epifluorescence microscopy (F420 autofluorescence) and in situ hybridization by using a newly designed M. mobile-specific 16S rRNA-targeted oligonucleotide probe found that methanogens accounted for approximately 3.6% of total ruminal microorganisms and approximately 54% of the total methanogens were M. mobile.

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© 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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