Intraspecific Divergence of Saccharomyces kluyveri as Revealed by the Nucleotide Sequences of 18S-28S rRNA Spacer Regions and α-Galactosidase MEL Genes

Abstract

In the five strains classified as the yeast Saccharomyces kluyveri, several substitutions were observed in the two internal transcribed spacer regions between 18S and 28S rRNA. A PCR reaction with primers targeted to the MEL1 gene of Saccharomyces cerevisiae amplified fragments of the expected size, and those sequences showed significant divergence in the strains of S. kluyveri.