Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology
Characterization of PBZ1, a Probenazole-inducible Gene, in Suspension-Cultured Rice Cells
Hideo NAKASHITAKeiko YOSHIOKAMasayuki TAKAYAMARie KUGANaoki MIDOHRon USAMIKoki HORIKOSHIKatsuyoshi YONEYAMAIsamu YAMAGUCHI
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2001 Volume 65 Issue 1 Pages 205-208

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Abstract

Probenazole (PBZ) induces non-race specific resistance in rice plants against rice blast fungus and PBZ1 was identified as a PBZ-inducible gene from rice. The induction of PBZ1 expression in suspension-cultured rice cells was investigated. Northern blot analysis indicated that PBZ1 was induced by PBZ in a dosedependent manner. Enzyme-linked immunosorbent assay (ELISA) showed a dose and time-dependent accumulation of PBZ1 protein. Both mRNA and protein analysis showed that PBZ1 was not induced by salicylic acid or an active metabolite, 1,2-benzisothiazole-1,1-dioxide.

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© 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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