Improvement of the Electro-transformation Efficiency of Facultatively Alkaliphilic Bacillus pseudofirmus OF4 by High Osmolarity and Glycine Treatment

抄録

An electro-transformation method was established for facultatively alkaliphilic Bacillus pseudofirmus OF4. A high osmolarity electroporation medium with a high electric field strength was effective. Transformation efficiency improved 110-fold in glycine-treated cells compared to non-glycine-treated cells. Under optimum conditions, the transformation efficiency was 1.69×10⁶ transformants per μg of pUB110.