A Simple and Rapid Method for Intra- and Interspecific Transformation of Bacillus subtilis on Solid Media by DNA in Protoplast Lysates

Abstract

A simple method for intra- and interspecific transformation of Bacillus subtilis on solid media has been devised with DNA in protoplast lysates, 0.8% agar, glutamate, and yeast extract. The transformation frequency is 2.3×10³ transformants per μg DNA, 10-20 times higher than that for conventional transformation on solid media. The method can be applicable to transformation in microtiter plates.