Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology
Piezoresponse of the cyo-Operon Coding for Quinol Oxidase Subunits in a Deep-sea Piezophilic Bacterium, Shewanella violacea
Kaoru NAKASONEMitsunori YAMADAMohammad Hassan QURESHIChiaki KATOKoki HORIKOSHI
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2001 年 65 巻 3 号 p. 690-693

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We have isolated the genes for quinol oxidase from a deep-sea piezophilic bacterium, Shewanella violacea. Analysis of the deduced amino acid sequences of the cyo subunits showed that this oxidase has high similarity to Escherichia coli bo-type quinol oxidase. Northern blot analysis showed that these genes are expressed at a high level when the bacterium is grown at elevated pressure. Upstream in the cyo-operon, a σ54-binding motif and an octamer sequence unit were found, suggesting that these elements may play a role in regulation of expression of the cyo-operon in response to changes in pressure.
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© 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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