Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660

Xiao-Yong ZHANG; An-Lan DAI; Kouji KUROIWA; Ritsuko KODAIRA; Masahiro NOGAWA; Makoto SHIMOSAKA; Mitsuo OKAZAKI

doi:10.1271/bbb.65.977

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology

Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660

Xiao-Yong ZHANG, An-Lan DAI, Kouji KUROIWA, Ritsuko KODAIRA, Masahiro NOGAWA, Makoto SHIMOSAKA, Mitsuo OKAZAKI

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Keywords: chitosanase, Aspergillus, gene cloning, koji mold

JOURNAL FREE ACCESS

2001 Volume 65 Issue 4 Pages 977-981

DOI https://doi.org/10.1271/bbb.65.977

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Abstract

A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A. oryzae csnA contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 Da. The deduced amino acid sequence of A. oryzae csnA indicates extensive similarities to those of other fungal chitosanases.

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