Co-existing Proteins Interfere with the Action of Nordihydroguaiaretic Acid on Retrograde Golgi-to-ER Protein Trafficking in NRK Cells and α-Glucosidase Reaction in vitro

Abstract

Induction of retrograde trafficking of mannosidase II and TGN38 in NRK cells and inhibition of α-glucosidase in vitro by nordihydroguaiaretic acid (NDGA) were strongly interfered with by serum, serum albumin, or other unrelated proteins added to the medium or incubation mixture. These observations indicate that NDGA interacts with diverse kinds of proteins, and therefore, pharmacological effects of NDGA at cellular levels should be carefully interpreted.