Co-existing Proteins Interfere with the Action of Nordihydroguaiaretic Acid on Retrograde Golgi-to-ER Protein Trafficking in NRK Cells and α-Glucosidase Reaction in vitro

Kazuhito SATO; Makoto MUROI; Machiko NAKAMURA; Makoto FUJIMURA; Akira TAKATSUKI

doi:10.1271/bbb.65.996

Biochemistry & Molecular Biology

Co-existing Proteins Interfere with the Action of Nordihydroguaiaretic Acid on Retrograde Golgi-to-ER Protein Trafficking in NRK Cells and α-Glucosidase Reaction in vitro

Kazuhito SATO, Makoto MUROI, Machiko NAKAMURA, Makoto FUJIMURA, Akira TAKATSUKI

著者情報

キーワード: nordihydroguaiaretic acid, retrograde trafficking, Golgi apparatus, α-glucosidase

ジャーナルフリー

2001 年 65 巻 4 号 p. 996-998

DOI https://doi.org/10.1271/bbb.65.996

詳細

抄録

Induction of retrograde trafficking of mannosidase II and TGN38 in NRK cells and inhibition of α-glucosidase in vitro by nordihydroguaiaretic acid (NDGA) were strongly interfered with by serum, serum albumin, or other unrelated proteins added to the medium or incubation mixture. These observations indicate that NDGA interacts with diverse kinds of proteins, and therefore, pharmacological effects of NDGA at cellular levels should be carefully interpreted.

責任著者(Corresponding author)

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