Evidence for the Presence of DNA-Binding Proteins Involved in Regulation of the Gene Expression of Indole-3-Pyruvic Acid Decarboxylase, a Key Enzyme in Indole-3-Acetic Acid Biosynthesis in Azospirillum lipoferum FS

Ken YAGI; Tetsuya CHUJO; Hideaki NOJIRI; Toshio OMORI; Makoto NISHIYAMA; Hisakazu YAMANE

doi:10.1271/bbb.65.1265

Biochemistry & Molecular Biology

Evidence for the Presence of DNA-Binding Proteins Involved in Regulation of the Gene Expression of Indole-3-Pyruvic Acid Decarboxylase, a Key Enzyme in Indole-3-Acetic Acid Biosynthesis in Azospirillum lipoferum FS

Ken YAGI, Tetsuya CHUJO, Hideaki NOJIRI, Toshio OMORI, Makoto NISHIYAMA, Hisakazu YAMANE

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Keywords: Azospirillum lipoferum FS, indole-3-pyruvic acid decarboxylase, indole-3-acetic acid biosynthesis, inverted repeat sequence, regulatory mechanism

JOURNAL FREE ACCESS

2001 Volume 65 Issue 5 Pages 1265-1269

DOI https://doi.org/10.1271/bbb.65.1265

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Abstract

We isolated the ipdc gene coding for indole-3-pyruvic acid decarboxylase (IPDC), a key enzyme in the indole-3-pyruvic acid pathway for indole-3-acetic acid biosynthesis, in the plant growth-promoting rhizobacterium Azospirillum lipoferum FS. Gel mobility-shift assay showed the presence of two DNA-binding proteins that might be involved in regulation of the ipdc gene expression.

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