Copper Staining Method for Extracting Biologically Active Proteins from Native Gels

Jie WEN; Mei ZHANG; Thomas P. HORAN; John S. PHILO; Tiansheng LI; Jette WYPYCH; Elizabeth A. MENDIAZ; Keith E. LANGLEY; Kenneth H. AOKI; Moeka KUWAMOTO; Yoshiko KITA; Tsutomu ARAKAWA

doi:10.1271/bbb.65.1315

Analytical Chemistry

Copper Staining Method for Extracting Biologically Active Proteins from Native Gels

Jie WEN, Mei ZHANG, Thomas P. HORAN, John S. PHILO, Tiansheng LI, Jette WYPYCH, Elizabeth A. MENDIAZ, Keith E. LANGLEY, Kenneth H. AOKI, Moeka KUWAMOTO, Yoshiko KITA, Tsutomu ARAKAWA

著者情報

キーワード: copper staining, native gel, non-denaturing, protein recovery, extraction of protein

ジャーナルフリー

2001 年 65 巻 6 号 p. 1315-1320

DOI https://doi.org/10.1271/bbb.65.1315

詳細

抄録

An attempt was made to make protein bands visible on native gel using copper staining, since such a mild staining procedure would make the entire native gel electrophoresis process non-denaturing. Copper staining not only was able to detect various proteins on native gel with reasonable sensitivity, but also made extraction and recovery of active proteins possible from the gel using a gentle procedure.

責任著者(Corresponding author)

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